Scientists have recovered the first genetic data from an extinct bird in the Caribbean, thanks to the remarkably preserved bones of a Creighton’s caracara from a flooded sinkhole on Great Abaco Island.
Studies of ancient DNA from tropical birds have faced two formidable obstacles. Organic material quickly degrades when exposed to heat, light and oxygen. And birds’ lightweight, hollow bones break easily, accelerating the decay of the DNA within.
But the dark, oxygen-free depths of a 100-foot blue hole known as Sawmill Sink provided ideal preservation conditions for the bones of Caracara creightoni, a species of large carrion-eating falcon that disappeared soon after humans arrived in the Bahamas about 1,000 years ago.
Florida Museum of Natural History postdoctoral researcher Jessica Oswald extracted and sequenced genetic material from a 2,500-year-old C. creightoni femur from the blue hole. Because ancient DNA is often fragmented or missing, Oswald had modest expectations for what she would find—maybe one or two genes. But instead, the bone yielded 98.7% of the bird’s mitochondrial genome, the set of DNA that most living things inherit only from their mothers.
“I was super excited. I would have been happy to get that amount of coverage from a fresh specimen,” said Oswald, lead author of a study describing the work and also a postdoctoral researcher at the University of Nevada, Reno. “Getting DNA from an extinct bird in the tropics is significant because it hasn’t been successful in many cases or even tried.”
The mitochondrial genome showed that C. creightoni is closely related to the two remaining caracara species alive today: the crested caracara, Caracara cheriway, and the southern caracara, Caracara plancus. The three species last shared a common ancestor between 1.2 and 0.4 million years ago.
At least six species of caracara once cleaned carcasses and picked off small prey in the Caribbean. But the retreat of glaciers 15,000 years ago and the resulting rise in sea levels triggered extinctions of many birds, said David Steadman, Florida Museum curator of ornithology.
“For birds, having an entire head of an extinct species from a fossil site is pretty mind-blowing,” Oswald said. “Because all the material from the blue hole is beautifully preserved, we thought at least some DNA would probably be there.”
Since 2017, Oswald has been revitalizing the museum’s ancient DNA laboratory, testing methods and developing best practices for extracting and analyzing DNA from fossils and objects that are hundreds to millions of years old.
Ancient DNA is a challenging medium because it’s in the process of degradation. Sometimes only a minute quantity of an animal’s original DNA—or no DNA at all—remains after bacteria, fungi, light, oxygen, heat and other environmental factors have broken down an organism.
“With ancient DNA, you take what you can get and see what works,” Oswald said. “Every bone has been subjected to slightly different conditions, even relative to other ones from the same site.”
To maximize her chance of salvaging genetic material, Oswald cleans a bone, freezes it with liquid nitrogen and then pulverizes it into powder with a rubber mallet.
“It’s pretty fun,” she said.
While previous studies required large amounts of bone, Oswald’s caracara work showed ancient DNA could be successfully recovered at a smaller scale.
“This puts an exclamation point on what’s possible with ancient DNA,” said Robert Guralnick, Florida Museum curator of bioinformatics. “We have new techniques for looking at the context of evolution and extinction. Beyond the caracara, it’s cool that we have an ancient DNA lab that’s going to deliver ways to look at questions not only from the paleontological perspective, but also at the beginnings of a human-dominated planet.”
Steadman, who has spent decades researching modern and extinct biodiversity in the Caribbean, said some questions can only be answered with ancient DNA.
“By understanding species that weren’t able to withstand human presence, it helps us better appreciate what we have left—and not just appreciate it, but understand that when these species evolved, there were a lot more things running and flying around than we have today.”
The researchers published their findings in Molecular Phylogenetics and Evolution.
Jessica A. Oswald et al, Ancient DNA from a 2,500-year-old Caribbean fossil places an extinct bird (Caracara creightoni) in a phylogenetic context, Molecular Phylogenetics and Evolution (2019). DOI: 10.1016/j.ympev.2019.106576
Extinct Caribbean bird yields DNA after 2,500 years in watery grave (2019, August 15)
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